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| 别名: | IL-23 alpha & IL-12 beta | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Mouse IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free
IL-23 alpha & IL-12 beta
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Freeis expressed from human 293 cells. It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B).
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequence
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 19 kDa (IL-23A) and 40-44 kDa (IL-12B) respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Immobilized Human IL-23 R, Fc Tag at 10 μg/mL (100 μL/well) can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Freewith a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
Loaded Mouse IL-23R, Fc Tagon Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 20.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-23 R, Fc Tagon Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Freewith an affinity constant of 11.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tagon Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 3.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.008-0.0134 ng/mL (Routinely tested).
