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| 别名: | IL1B,IL-1BETA,IL1F2,IL-1β | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-1 beta / IL-1F2 Protein, Tag Free (MALS verified)
IL1B,IL-1BETA,IL1F2,IL-1β
Human IL-1 beta, Tag Free is expressed from E.coli cells. It contains AA Ala 117 - Ser 269.
Predicted N-terminus: Met
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 17.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
Less than 0.1 EU per μg by the LAL method.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-1 beta, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-1 beta, Tag Free is more than 95% and the molecular weight of this protein is around 15-23 kDa verified by SEC-MALS.
Report
Immobilized Human IL-1 beta, Tag Freeat 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tagwith a linear range of 8-63 ng/mL (QC tested).
Protocol
Human IL-1 RII, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, Tag Free with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human IL-1 beta, Tag Freestimulates secretion of IFN-gamma by NK-92 human natural killer lymphoma cells. The EC50 for this effect is 1.02-1.33 ng/mL (Routinely tested).
