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Human GM-CSF R alpha Protein, Fc Tag

Human GM-CSF R alpha Protein, Fc Tag
别名: GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,CSF2RA,CSF2R,CSF2RY,CDw116,CD116
储存条件: -20℃ 
Human GM-CSF R alpha Protein, Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GRA-H5255-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

Human GM-CSF R alpha Protein, Fc Tag

分子别名(Synonym)

GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,CSF2RA,CSF2R,CSF2RY,CDw116,CD116

表达区间及表达系统(Source)

Human GM-CSF R alpha, Fc Tag is expressed from human 293 cells. It contains AA Glu 23 - Gly 320.

Predicted N-terminus: Glu 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 60.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human GM-CSF R alpha, Fc Tag (Cat. No. GRA-H5255) SDS-PAGE gel

Human GM-CSF R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GM-CSF, Tag Freeat 1 μg/mL (100 μL/well) can bind Human GM-CSF R alpha, Fc Tagwith a linear range of 0.5-16 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human GM-CSF R alpha, Fc Tag n Protein A Biosensor, can bind ActiveMax® Human GM-CSF, Tag Free  with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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