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Canine GITR / TNFRSF18 Protein, His Tag

Canine GITR / TNFRSF18 Protein, His Tag
别名: AITR,GITR,TNFRSF18,CD357
储存条件: -20℃ 
Canine GITR / TNFRSF18 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GIR-C52H6-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Canine GITR / TNFRSF18 Protein, His Tag

分子别名(Synonym)

AITR,GITR,TNFRSF18,CD357

表达区间及表达系统(Source)

Canine GITR, His Tag  is expressed from human 293 cells ). It contains AA Gly 23 - Pro 154.

Predicted N-terminus: Gly 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.8 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Canine GITR, His Tag (Cat. No. GIR-C52H6) SDS-PAGE gel

Canine GITR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Canine GITR, His Tag  at 2 μg/mL (100 μL/well) can bind Human GITR Ligand, Fc Tag with a linear range of 2-40 ng/mL (QC tested).

 

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