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Human M-CSF R / CSF1R / CD115 Protein, Mouse IgG2a Fc Tag (MALS verified)

Human M-CSF R / CSF1R / CD115 Protein, Mouse IgG2a Fc Tag (MALS verified)
别名: CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
储存条件: -20℃ 
Human M-CSF R / CSF1R / CD115 Protein, Mouse IgG2a Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CSR-H5255-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

Human M-CSF R / CSF1R / CD115 Protein, Mouse IgG2a Fc Tag (MALS verified)

分子别名(Synonym)

CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR

表达区间及表达系统(Source)

Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified)  is expressed from human 293 cells. It contains AA Ile 20 - Glu 512 

Predicted N-terminus: Ile 20

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 81.4 kDa. The protein migrates as 105-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human M-CSF R,  Mouse IgG2a Fc Tag (MALS verified) (Cat. No. CSR-H5255) SDS-PAGE gel

Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified)  is more than 85% and the molecular weight of this protein is around 210-230 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified)  at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag  with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified) t 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-34, His,Avitag with a linear range of 0.2-10 ng/mL (Routinely tested).

 

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