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| 别名: | DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human TNF-alpha Protein, His Tag (active trimer) (MALS verified)
DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
Human TNF-alpha, His Tag (active trimer) (MALS verified) is expressed from human 293 cells . It contains AA Val 77 - Leu 233 .
Predicted N-terminus: Val 77
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.3 kDa. The protein migrates as 18.2 kDa under reducing (R) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human TNF-alpha, His Tag (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human TNF-alpha, His Tag (active trimer) (MALS verified) is more than 95% in HP-SEC, and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Immobilized Human TNF-alpha, His Tag at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag ith a linear range of 0.4-13 ng/mL (QC tested).
Protocol
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, His Tag (active trimer) (MALS verified) (Cat. No. TNA-H5228) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human TNF-alpha, His Tag (active trimer) (MALS verified) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.013-0.074 ng/mL (Routinely tested).
Protocol
Neutralization assay shows that the cytotoxicity effect of Human TNF-alpha, His Tag (active trimer) (MALS verified)was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 26.8 ng/mL (Routinely tested).
