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Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)
别名: IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG
储存条件: -20℃ 
Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILG-M5254-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

分子别名(Synonym)

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统(Source)

Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag is expressed from human 293 cells. It contains AA Ala 27 - Glu 240 (IL-2RB) & Trp 23 - Ala 263 (IL-2RG).

Predicted N-terminus: Ala 27 (IL-2RB) & Trp 23 (IL-2RG)

Request for sequence

蛋白结构(Molecular Characterization)

Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 51.5 kDa (IL-2RB) and 54.5 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag  (Cat. No. ILG-M5254) SDS-PAGE gel

Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag  is more than 85% and the molecular weight of this protein is around 138-168 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human IL-2, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag with a linear range of 0.008-0.25 μg/mL (QC tested).

 

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