-
专业包装 正品保证
-
快乐服务 售后无忧
-
会员特权 优惠不断
-
个人信息 严格保护
| 别名: | IL4,BCGF1,BSF1 | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human IL-4 Protein, Avitag™,His Tag (MALS verified)
IL4,BCGF1,BSF1
Biotinylated Human IL-4, Avitag,His Tag is expressed from human 293 cells . It contains AA His 25 - Ser 153
Predicted N-terminus: His 25
Request for sequence
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 17.6 kDa. As a result of glycosylation, the protein migrates as 22 kDa under reducing (R) condition, and 21 kDa under non-reducing (NR) condition (SDS-PAGE).
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-4, Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human IL-4, Avitag,His Tag is more than 85% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.
Report
Immobilized Human IL-4 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag,His Tag with a linear range of 2-16 ng/mL (QC tested).
Protocol
Immobilized Human IL-4 R alpha, Fc Tag at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, His Tag Biotinylated Human IL-4, Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.505 μg/mL (Routinely tested).
Protocol
Biotinylated Human IL-4, Avitag,His Tag stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.19-2.64 ng/mL (Routinely tested).
