Biotinylated Human IL-4 Protein, Avitag™,His Tag (MALS verified)

Biotinylated Human IL-4 Protein, Avitag™,His Tag (MALS verified)

分子别名（Synonym）

IL4,BCGF1,BSF1

表达区间及表达系统（Source）

Biotinylated Human IL-4, Avitag,His Tag is expressed from human 293 cells . It contains AA His 25 - Ser 153

Predicted N-terminus: His 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 17.6 kDa. As a result of glycosylation, the protein migrates as 22 kDa under reducing (R) condition, and 21 kDa under non-reducing (NR) condition (SDS-PAGE).

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human IL-4, Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

The purity of Biotinylated Human IL-4, Avitag,His Tag is more than 85% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human IL-4 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag,His Tag with a linear range of 2-16 ng/mL (QC tested).

Protocol

Immobilized Human IL-4 R alpha, Fc Tag at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, His Tag Biotinylated Human IL-4, Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.505 μg/mL (Routinely tested).

Protocol

活性（Bioactivity）-Cell based assay

Biotinylated Human IL-4, Avitag,His Tag stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.19-2.64 ng/mL (Routinely tested).

 

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！