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| 别名: | FLT,VEGFR1,FLT1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human VEGF R1 / Flt-1 Protein, His Tag (MALS verified)
RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
ActiveMax® Human VEGF165, Tag Free (MALS verified) is expressed from human 293 cells . It contains AA Ala 27 - Arg 191 .
Predicted N-terminus: Ala 27
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 19.2 kDa. As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>98% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70°C for 24 months under sterile conditions after reconstitution.
ActiveMax® Human VEGF165, Tag Free (MALS verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
The purity of ActiveMax® Human VEGF165, Tag Free (MALS verified) is more than 95% in HP-SEC, and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
Immobilized ActiveMax® Human VEGF165, Tag Free (MALS verified) at 0.01 μg/mL (100 μL/well) can bind Human VEGF R1, His Tag with a linear range of 4-31 ng/mL (QC tested).
Protocol
Immobilized ActiveMax® Human VEGF165, Tag Free (MALS verified) at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R2, Avitag,His Tag with a linear range of 10-156 ng/mL (Routinely tested).
Protocol
Immobilized ActiveMax® Human VEGF165, Tag Free (MALS verified) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 μg/mL (100 μL/well) Biotinylated Human VEGF R2, Avitag,His Tag with a half maximal inhibitory concentration (IC50) of 0.70 μg/mL (Routinely tested).
Protocol
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human VEGF165, Tag Free (MALS verified)with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind ActiveMax® Human VEGF165, Tag Free (MALS verified)with an affinity constant of 0.016 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human NRP1, Fc Tagon Protein A Biosensor, can bind Human VEGF165, Tag Free with an affinity constant of 12.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human VEGF R1, His Tag on HIS1K Biosensor, can bind ActiveMax® Human VEGF165, Tag Free with an affinity constant of 43.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
ActiveMax® Human VEGF165, Tag Free (MALS verified) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/mL (Routinely tested).
Protocol
Inhibition assay shows that the proliferation effect of ActiveMax® Human VEGF165, Tag Free (MALS verified) is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/mL. The ED50 is 0.065-0.229 μg/mL (Routinely tested).
