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ClinMax™Human Interleukin 8 (IL-8) Quantitative ELISA Kit
ClinMax™Human IL-8 Quantitative ELISA Kit is designed to quantitate human IL-8 that is present in complex biological matrices, such as human serum, plasma, buffered solution, or cell culture. Interleukin 8 (IL-8) is a chemotactic factor that attracts neutrophils, basophils, and T-cells during the inflammatory process. Like all chemokines, IL-8 is a small, soluble peptide (8–10 kDa).Interleukin-8 is synthesized primarily as a 99 amino acid peptide that is processed through signal sequence cleavage and N-terminal proteolysis to yield several active IL-8 isoforms , with peptides of 72 and 77 amino acids being most biologically relevant. The 72 amino acid peptide is the major form secreted by monocytes and macrophages in culture, whereas the 77 amino acid variant is the most abundant secretory product of nonimmune cells.
This immunoassay is designed to quantitate human IL-8 that is present in human serum, plasma, buffered solution, or cell culture. It has been calibrated against a highly purified human IL-8 and is evaluated with standard from NIBSC/WHO. Reference Reagent Interleukin-8 (Human, rDNA derived) NIBSC code: 89/520.
It is for research use only.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.