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| 别名: | DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
ActiveMax® Human TNF-alpha Protein, Tag Free, low endotoxin (active trimer) (MALS verified)
DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
ActiveMax® Human TNF-alpha, Tag Frees expressed from human 293 cells . It contains AA Val 77 - Leu 233 .
Predicted N-terminus: Val 77
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
Less than 0.1 EU per μg by the LAL method.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human TNF-alpha, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified)is more than 95% in HP-SEC, and the molecular weight of this protein is around 47-60 kDa verified by SEC-MALS.
Report
Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified)at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin t 5 μg/mL (100 μL/well) can bind Human TNFR2, His Tag with a linear range of 5-39 ng/mL (Routinely tested).
Protocol
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).
Protocol
Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).
