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| 别名: | EGF,URG,HOMG4 | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human EGF Protein, Mouse IgG2a Fc Tag
EGF,URG,HOMG4
Human EGF, Mouse IgG2a Fc Tagis expressed from human 293 cells). It contains AA Asn 971 - Arg 1023 .
Predicted N-terminus: Glu
Request for sequence
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 33.1 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human EGF, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human EGF R, Fc Tag at 5 μg/mL (100 μL/well)can bind Human EGF, Mouse IgG2a Fc Tag with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
Human EGF R, His Tag captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag with an affinity constant of 2.63 nM as determined in SPR assay (Biacore T200) (Routinely tested).
