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Human VEGF-B Protein, His Tag

Human VEGF-B Protein, His Tag
别名: VRF,VEGFL,VEGFB,VEGF-B
储存条件: -20℃ 
Human VEGF-B Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-VE6-H5225-20ug 20ug 现货 1800.00 1800
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熔点:
密度:
储存条件: -20℃

 

Human VEGF-B Protein, His Tag

分子别名(Synonym)

VRF,VEGFL,VEGFB,VEGF-B

表达区间及表达系统(Source)

Human VEGF-B, His Tag  is expressed from human 293 cells . It contains AA Pro 22 - Ala 207 .

Predicted N-terminus: Pro 22

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.3 kDa. The protein migrates as 16 kDa and 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human VEGF-B, His Tag (Cat. No. VE6-H5225) SDS-PAGE gel

Human VEGF-B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Measured by its binding ability in a functional ELISA. Immobilized Human VEGF-B, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1 with a linear range of 2-32 ng/mL (QC tested).

 

 

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