Biotinylated Human TGF-Beta 1 / TGFB1 Protein, Avitag™

 Biotinylated Human TGF-Beta 1 / TGFB1 Protein, Avitag™

分子别名（Synonym）

CEDLAP,TGF-beta 1,TGFB1,DPD1,TGF-beta-1,TGFB

表达区间及表达系统（Source）

Biotinylated Human TGF-Beta 1, Avitag  is expressed from human 293 cells. It contains AA Ala 279 - Ser 390

Predicted N-terminus: Ala 279

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries an Avi tag at the C-terminus.

The protein has a calculated MW of 14.5 kDa. As a result of glycosylation, the protein migrates as 14-15 kDa under reducing (R) condition, and 24-25 kDa under non-reducing (NR) condition (SDS-PAGE).

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM HAC, pH2.9. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.
This product does not contains polyhis tag, but there exists extremely low level of polyhis tag which might interfere with your assay when using together with anti-his tag secondary antibody.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 24 months in lyophilized state;

2. -70°C for 12 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human TGF-Beta 1, Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized Human TGFBR2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TGF-Beta 1, Avitag with a linear range of 0.1-3 ng/mL (QC tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！