-
专业包装 正品保证
-
快乐服务 售后无忧
-
会员特权 优惠不断
-
个人信息 严格保护
| 别名: | FLT,VEGFR1,FLT1 | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human VEGF R1 / Flt-1 Protein, His,Avitag™
FLT,VEGFR1,FLT1
Biotinylated Human VEGF R1, His,Avitag is expressed from human 293 cells . It contains AA Ser 27 - Asn 756
Predicted N-terminus: Ser 27
Request for sequence
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 85.9 kDa. The protein migrates as 106-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human VEGF R1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human VEGF165, Tag Free at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized Human PLGF, His Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitaith a linear range of 2-20 ng/mL (Routinely tested).
Protocol
Loaded Biotinylated Human VEGF R1, His,Avitag on SA Biosensor, can bind Human VEGF165, His Tag with an affinity constant of 0.236 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
