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| 别名: | IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human IL-3 R alpha / CD123 Protein, Fc,Avitag™ (MALS verified)
IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha
Biotinylated Human IL-3 R alpha, Fc,Avitag is expressed from human 293 cells It contains AA Thr 19 - Arg 305
Predicted N-terminus: Thr 19
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.2 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-3 R alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human IL-3 R alpha, Fc,Avitag is more than 90% and the molecular weight of this protein is around 128-156 kDa verified by SEC-MALS.
Report
Immobilized Anti-IL-3 R alpha Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-3 R alpha, Fc,Avitag with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-3 R alpha, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidinprecoated (0.5 μg/well) plate can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.6-5 ng/mL (Routinely tested).
Protocol
Loaded Biotinylated Human IL-3 R alpha, Fc,Avitag on SA Biosensor, can bind Human IL-3, His Tagwith an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
