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| 别名: | GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,CSF2RA,CSF2R,CSF2RY,CDw116,CD116 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human GM-CSF R alpha Protein, His Tag (SPR verified)
GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,CSF2RA,CSF2R,CSF2RY,CDw116,CD116
Human GM-CSF R alpha, His Tag is expressed from human 293 cells . It contains AA Glu 23 - Gly 320.
Predicted N-terminus: Glu 23
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human GM-CSF R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated Human GM-CSF, epitope tag free, primary amine labeling at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate can bind Human GM-CSF R alpha, His Tag with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, Tag Free with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested).
Protocol
Loaded Biotinylated Human GM-CSF, epitope tag free, primary amine labeling on SA Biosensor, can bind Human GM-CSF R alpha, His Tag with an affinity constant of 23.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
