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| 别名: | GM-CSF,CSF2,MGC131935 | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
ActiveMax® Human GM-CSF Protein, Tag Free
GM-CSF,CSF2,MGC131935
ActiveMax® Human GM-CSF, Tag Free is expressed from human 293 cells . It contains AA Ala 18 - Glu 144
Predicted N-terminus: Ala 18
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 14.5 kDa. The protein migrates as 18-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human GM-CSF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized ActiveMax® Human GM-CSF, Tag Free at 1 μg/mL (100 μL/well) can bind Human GM-CSF R alpha, Fc Tag with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Loaded Human GM-CSF R alpha, Fc Tagon Protein A Biosensor, can bind ActiveMax® Human GM-CSF, Tag Free with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.1 ng/mL, corresponding to a specific activity of > 1x107 Unit/mg.
