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| 别名: | Interferon-gamma ,Interferon-γ | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
ActiveMax® Human IFN-gamma / IFNG Protein, Tag Free
Interferon-gamma ,Interferon-γ
ActiveMax® Human IFN-gamma, Tag Free is expressed from human 293 cells. It contains AA Gln 24 - Gln 166 .
Predicted N-terminus: Gln 24
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 16.8 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human IFN-gamma, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax® Human IFN-gamma, Tag Free at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag with a linear range of 1-10 ng/mL (QC tested).
Protocol
The bio-activity of ActiveMax® Human IFN-gamma, Tag Free was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL (Routinely tested).
