Human Complement C5 Protein, His Tag

Human Complement C5 Protein, His Tag

分子别名（Synonym）

Complement C5,C5,CPAMD4

表达区间及表达系统（Source）

Human Complement C5, His Tagis expressed from human 293 cells . It contains AA Gln 19 - Cys 1676 

Predicted N-terminus: Gln 19 (β chain) & Thr 678 (α chain)

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.4 kDa (α chain). The protein migrates as 67 kDa (β chain) and 106-116 kDa (α chain) under reducing (R) condition (SDS-PAGE), and 130-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 24 months in lyophilized state;

2. -70°C for 12 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human Complement C5, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized Human Complement C5, His Tag  at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.2-3 ng/mL (QC tested).

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