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| 别名: | Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FLT-41,FLT41,PCL | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human VEGF R3 / FLT4 Protein, Fc Tag (MALS verified)
Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FLT-41,FLT41,PCL
Human VEGF R3, Fc Tags expressed from human 293 cells . It contains AA Tyr 25 - Ile 776 .
Predicted N-terminus: Tyr 25
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) condition, and 280-320 kDa under non-reducing (NR) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human VEGF R3, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human VEGF R3, Fc Tag is more than 85% and the molecular weight of this protein is around 260-320 kDa verified by SEC-MALS.
Report
Immobilized Human VEGF-C, His Tag at 5μg/mL (100 μL/well) can bind Human VEGF R3, Fc Tag with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
Immobilized Human VEGF R3, Fc Tag at 5μg/mL (100 μL/well) can bind Human VEGF-D, His Tag with a linear range of 0.08-0.6 μg/mL (Routinely tested).
Protocol
Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human VEGF-C, His Tag captured on CM5 Chip via anti-His antibody can bind Human VEGF R3, Fc Tag with an affinity constant of 41.1 pM as determined in SPR assay (Biacore T200) (Routinely tested).
