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Human IFN-alpha/beta R2 Protein, His Tag (MALS verified)

Human IFN-alpha/beta R2 Protein, His Tag (MALS verified)
别名: IFNAR2,IFNARB,IFNABR,IFN-R-2,IFN-alpha,beta receptor 2
储存条件: -20℃ 
Human IFN-alpha/beta R2 Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IF2-H5224-100ug 100ug 现货 2600.00 2600
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熔点:
密度:
储存条件: -20℃

Human IFN-alpha/beta R2 Protein, His Tag (MALS verified)

分子别名(Synonym)

IFNAR2,IFNARB,IFNABR,IFN-R-2,IFN-alpha,beta receptor 2

表达区间及表达系统(Source)

Human IFNAR2, His Tag is expressed from human 293 cells. It contains AA Ile 27 - Lys 243 .

Predicted N-terminus: Ile 27

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.8 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IFNAR2, His Tag (Cat. No. IF2-H5224) SDS-PAGE gel

Human IFNAR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IFNAR2, His Tag is more than 85% and the molecular weight of this protein is around 32-42 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IFN-alpha 2b (K46R), Fc Tag  immobilized on CM5 Chip can bind Human IFNAR2, His Tag  with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IFNAR2, His Tagn HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tagwith an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

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