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| 别名: | IL2RB,RP5-1170K4.6,CD122,P70-75 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-2 R beta / CD122 Protein, Fc Tag (MALS & SPR verified)
IL2RB,RP5-1170K4.6,CD122,P70-75
Human IL-2 R beta, Fc Tag is expressed from human 293 cells . It contains AA Ala 27 - Asp 239.
Predicted N-terminus: Ala 27
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.0 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-2 R beta, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-2 R beta, Fc Tag is more than 85% and the molecular weight of this protein is around 120-135 kDa verified by SEC-MALS.
Report
Immobilized Human IL-15, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tagwith a linear range of 5-156 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human IL-2, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-2 R beta, Fc Tag with a linear range of 0.3-2 ng/mL (Routinely tested).
Protocol
Human IL-2 R beta, Fc Tag captured on CM5 chip via anti-human IgG Fc antibody can bind IL-2, Tag Free with an affinity constant of 0.473 μM as determined in a SPR assay (Biacore T200) (QC tested).
