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Mouse EGF R Protein, His Tag

Mouse EGF R Protein, His Tag
别名: EGFR,ERBB,ERBB1,HER1,PIG61,mENA
储存条件: -20℃ 
Mouse EGF R Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-EGR-M5224-50ug 50ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Mouse EGF R Protein, His Tag

分子别名(Synonym)

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统(Source)

Mouse EGF R, His Tag  is expressed from human 293 cells . It contains AA Leu 25 - Ser 647.

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 71.2 kDa. The protein migrates as 86-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse EGF R, His Tag (Cat. No. EGR-M5224) SDS-PAGE gel

Mouse EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse EGF, Fc Tag  at 2 μg/mL (100 μL/well) can bind Mouse EGF R, His Tagwith a linear range of 0.02-0.313 μg/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Captured Mouse EGF R, His Tag on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200) (Routinely tested).

 

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