Human IGF-II Protein, Fc Tag

Human IGF-II Protein, Fc Tag

分子别名（Synonym）

IGF2,C11orf43,FLJ22066,FLJ44734,IGF-II,PP9974

表达区间及表达系统（Source）

Human IGF-II, Fc Tag  is expressed from human 293 cells . It contains AA Ala 25 - Glu 91 .

 

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蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 34.4 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human IGF-II, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized Human IGFBP-3, His Tag at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag with a linear range of 1.2-39 ng/mL (QC tested).

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