FITC-Labeled Human IL-15 R alpha / CD215 Protein, Fc Tag (MALS verified)

FITC-Labeled Human IL-15 R alpha / CD215 Protein, Fc Tag (MALS verified)

分子别名（Synonym）

IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha

表达区间及表达系统（Source）

FITC-Labeled Human IL-15 R alpha, Fc Tag is expressed from human 293 cells . It contains AA Ile 31-Thr 205 .

Predicted N-terminus: Ile 31

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 44.8 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记（Labeling）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

蛋白标记度（FITC:Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

FITC-Labeled Human IL-15 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of FITC-Labeled Human IL-15 R alpha, Fc Tag is more than 85% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human IL-15, His Tag at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human IL-15 R alpha, Fc Tag with a linear range of 0.078-5 μg/mL (QC tested).

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