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| 别名: | IL4R,CD124,IL4RA | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-4 R alpha / CD124 Protein, Fc Tag (MALS verified)
IL4R,CD124,IL4RA
Human IL-4 R alpha, Fc Tag is expressed from human 293 cells . It contains AA Met 26 - His 232
Predicted N-terminus: Met 26
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.4 kDa. The protein migrates as 57-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-4 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-4 R alpha, Fc Tag is more than 90% and the molecular weight of this protein is around 113-138 kDa verified by SEC-MALS.
Report
Immobilized Human IL-4, Tag Free at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human IL-4 R alpha, Fc Tag at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, Fc Tag: Biotinylated Human IL-4, Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 1.144 μg/mL (Routinely tested).
