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Human FGF acidic Protein, Tag Free

Human FGF acidic Protein, Tag Free
别名: FGF1,AFGF,ECGF,ECGF-beta,ECGFA,ECGFB,FGF-alpha,FGFA,GLIO703,HBGF1
储存条件: -20℃ 
Human FGF acidic Protein, Tag Free
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-AFF-H4116-200ug 200ug 现货 1800.00 1800
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熔点:
密度:
储存条件: -20℃

 

Human FGF acidic Protein, Tag Free

分子别名(Synonym)

FGF1,AFGF,ECGF,ECGF-beta,ECGFA,ECGFB,FGF-alpha,FGFA,GLIO703,HBGF1

表达区间及表达系统(Source)

Human FGF acidic, Tag Free is expressed from E.coli cells. It contains AA Phe 16 - Asp 155 .

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries no "tag".

The protein has a calculated MW of 16.0 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human FGF acidic, Tag Free (Cat. No. AFF-H4116) SDS-PAGE gel

Human FGF acidic, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF acidic, Tag Free  with an affinity constant of 186 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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