Human IL-15 Protein, Tag Free (SPR verified)_细胞生物学_生命科学

Human IL-15 Protein, Tag Free (SPR verified)

别名:	IL-15,Interleukin-15,MGC9721
		储存条件:	-20℃

货号	规格	可用库存	销售价(RMB)	您的折扣价(RMB)	购买数量
ZY-IL5-H4117-20ug	20ug	现货	~~1980.00~~	1980

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熔点:
密度:
储存条件:	-20℃

Human IL-15 Protein, Tag Free (SPR verified)

分子别名（Synonym）

IL-15,Interleukin-15,MGC9721

表达区间及表达系统（Source）

Human IL-15, Tag Free is expressed from E.coli cells. It contains AA Asn 49 - Ser 162.

Predicted N-terminus: Met

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-15, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human IL-15, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tagwith a linear range of 0.6-39 ng/mL (QC tested).

Protocol

活性（Bioactivity）-SPR

Human IL-2 R beta, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Human IL-2 R beta, Fc Tag (SPR verified)n Protein A Biosensor, can bind Human IL-15, Tag Freewith an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

活性（Bioactivity）-Cell based assay

Human IL-15, Tag Free stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.097-0.19 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

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标准品

Human IL-15 Protein, Tag Free (SPR verified)

分子别名（Synonym）

IL-15,Interleukin-15,MGC9721

表达区间及表达系统（Source）

Human IL-15, Tag Free is expressed from E.coli cells. It contains AA Asn 49 - Ser 162.

Predicted N-terminus: Met

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-15, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human IL-15, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tagwith a linear range of 0.6-39 ng/mL (QC tested).

Protocol

活性（Bioactivity）-SPR

Human IL-2 R beta, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Human IL-2 R beta, Fc Tag (SPR verified)n Protein A Biosensor, can bind Human IL-15, Tag Freewith an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

活性（Bioactivity）-Cell based assay

Human IL-15, Tag Free stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.097-0.19 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

精细化学

分析色谱

材料科学

生命科学

标准品

Human IL-15 Protein, Tag Free (SPR verified)

分子别名（Synonym）

IL-15,Interleukin-15,MGC9721

表达区间及表达系统（Source）

Human IL-15, Tag Free is expressed from E.coli cells. It contains AA Asn 49 - Ser 162. Predicted N-terminus: Met Request for sequence

蛋白结构（Molecular Characterization）

This protein carries no "tag". The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-15, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human IL-15, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tagwith a linear range of 0.6-39 ng/mL (QC tested). Protocol

活性（Bioactivity）-SPR

Human IL-2 R beta, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested). Protocol

活性（Bioactivity）-BLI

Loaded Human IL-2 R beta, Fc Tag (SPR verified)n Protein A Biosensor, can bind Human IL-15, Tag Freewith an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Protocol

活性（Bioactivity）-Cell based assay

Human IL-15, Tag Free stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.097-0.19 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

Human IL-15, Tag Free is expressed from E.coli cells. It contains AA Asn 49 - Ser 162.

Predicted N-terminus: Met

Request for sequence

This protein carries no "tag".

The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

Immobilized Human IL-15, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tagwith a linear range of 0.6-39 ng/mL (QC tested).

Protocol

Human IL-2 R beta, Fc Tag (SPR verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Loaded Human IL-2 R beta, Fc Tag (SPR verified)n Protein A Biosensor, can bind Human IL-15, Tag Freewith an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol