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Human IL-17 RA / CD217 Protein, Fc Tag (MALS verified)

Human IL-17 RA / CD217 Protein, Fc Tag (MALS verified)
别名: CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R
储存条件: -20℃ 
Human IL-17 RA / CD217 Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILA-H5257-200ug 200ug 现货 2600.00 2600
熔点:
密度:
储存条件: -20℃

 

Human IL-17 RA / CD217 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R

表达区间及表达系统(Source)

Human IL-17 RA, Fc Tag (MALS verified)  is expressed from human 293 cells. It contains AA Leu 33 - Trp 320.

Predicted N-terminus: Leu 33

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 60.1 kDa. The protein migrates as 71-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-17 RA, Fc Tag (MALS verified) (Cat. No. ILA-H5257) SDS-PAGE gel

Human IL-17 RA, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IL-17 RA, Fc Tagis more than 85% and the molecular weight of this protein is around 150-170 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-17F, His Tag  at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tagwith a linear range of 5-156 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human IL17A, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 0.5-4 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL17A, Tag Free with an affinity constant of 0.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag with an affinity constant of 34.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tagon Protein A Biosensor, can bind Human IL-17F  His Tag with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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