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Human BAFFR / TNFRSF13C Protein, Fc Tag

Human BAFFR / TNFRSF13C Protein, Fc Tag
别名: BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R
储存条件: -20℃ 
Human BAFFR / TNFRSF13C Protein, Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-BAR-H5257-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human BAFFR / TNFRSF13C Protein, Fc Tag

分子别名(Synonym)

BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R

表达区间及表达系统(Source)

Human BAFFR, Fc Tagis expressed from human 293 cells. It contains AA Ser 7 - Ala 71

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 33.2 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human BAFFR, Fc Tag (Cat. No. BAR-H5257) SDS-PAGE gel

Human BAFFR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human BAFF, His Tag at 5 μg/mL (100 μL/well) can bind Human BAFFR, Fc Tagwith a linear range of 2-31 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

Biotinylated Human ELISA

Immobilized Human BAFFR, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag: Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) binding reactions. The half maximal inhibitory concentration (IC50) is 0.7363 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Immobilized Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified)  on SA Chip can bind Human BAFFR, Fc Tag with an affinity constant of 26.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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