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Cynomolgus IL-17F Protein, His Tag

Cynomolgus IL-17F Protein, His Tag
别名: IL-17F,Interleukin-17F,Cytokine ML-1
储存条件: -20℃ 
Cynomolgus IL-17F Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILF-C5243-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Cynomolgus IL-17F Protein, His Tag

分子别名(Synonym)

IL-17F,Interleukin-17F,Cytokine ML-1

表达区间及表达系统(Source)

Cynomolgus IL-17F, His Tag is expressed from human 293 cells. It contains AA Arg 31 - Gln 163 .

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) SDS-PAGE gel

Cynomolgus IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus IL-17F, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tagwith a linear range of 0.005-0.313 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Cynomolgus IL-17F, His Tag  at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-17RC Isoform 1 Fc Chimera with a linear range of 0.5-16 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag  on Protein A Biosensor, can bind Cynomolgus IL-17F, His Tag with an affinity constant of 279 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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