Canine TNF-alpha Protein, His Tag, active trimer (MALS verified)

Canine TNF-alpha Protein, His Tag, active trimer (MALS verified)

分子别名（Synonym）

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表达区间及表达系统（Source）

Canine TNF-alpha, His Tag  is expressed from human 293 cells. It contains AA Val 77 - Leu 233 .

Predicted N-terminus: Val 77

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Canine TNF-alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Canine TNF-alpha, His Tag is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag with a linear range of 0.3-1 ng/mL (QC tested).

Protocol

Immobilized Human TNFR1, Fc Tag at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag  with a linear range of 2-10 ng/mL (Routinely tested).

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