Biotinylated Human VEGF R2 / KDR Protein, Avitag™,His Tag

Biotinylated Human VEGF R2 / KDR Protein, Avitag™,His Tag

分子别名（Synonym）

KDR,CD309,FLK1,VEGFR,VEGFR2

表达区间及表达系统（Source）

Biotinylated Human VEGF R2, Avitag,His Tagis expressed from human 293 cells It contains AA Ala 20 - Glu 764.

Predicted N-terminus: Ala 20

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 85.9 kDa. The protein migrates as 120-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human VEGF R2, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified)  at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R2, Avitag,His Tagwith a linear range of 10-156 ng/mL (QC tested).

Protocol

Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified)  at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 μg/mL (100 μL/well) Biotinylated Human VEGF R2, Avitag,His Tag with a half maximal inhibitory concentration (IC50) of 0.70 μg/mL (Routinely tested).

Protocol

活性（Bioactivity）-BLI

Loaded Biotinylated Human VEGF R2, Avitag,His Tagon SA Biosensor, can bind Human VEGF-D, His Tag  with an affinity constant of 482 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性（Bioactivity）-FACS

2e5 of anti-VEGF R2 CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human VEGF R2, Avitag,His Tag and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

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