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Human IFN-alpha 1 Protein, His Tag

Human IFN-alpha 1 Protein, His Tag
别名: IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D
储存条件: -20℃ 
Human IFN-alpha 1 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IFA-H52H9-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human IFN-alpha 1 Protein, His Tag

分子别名(Synonym)

IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D

表达区间及表达系统(Source)

Human IFN-alpha 1, His Tag  is expressed from human 293 cells. It contains AA Cys 24 - Glu 189

Predicted N-terminus: Cys 24

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) SDS-PAGE gel

Human IFN-alpha 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag with an affinity constant of 19.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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