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Human FGF19 Protein, His Tag

Human FGF19 Protein, His Tag
别名: FGF19,FGF-19,Fibroblast growth factor 19
储存条件: -20℃ 
Human FGF19 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-FG9-H51H3-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Human FGF19 Protein, His Tag

分子别名(Synonym)

FGF19,FGF-19,Fibroblast growth factor 19

表达区间及表达系统(Source)

Human FGF19, His Tag s expressed from E.coli cells. It contains AA Leu 25 - Lys 216.

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.4 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.3 M NaCl, 0.3 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human FGF19, His Tag (Cat. No. FG9-H51H3) SDS-PAGE gel

Human FGF19, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human FGF R4, Fc Tag on Protein A Biosensor, can bind Human FGF19, His Tagwith an affinity constant of 63.5 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

Biotinylated Human BLI

Loaded Human FGF19, His Tag on HIS1K Biosensor, can bind Human FGF R4, Fc Tag  with an affinity constant of 16.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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