Biotinylated Human IL-1 alpha / IL-1F1 Protein, His,Avitag™ (MALS verified)

Biotinylated Human IL-1 alpha / IL-1F1 Protein, His,Avitag™ (MALS verified)

分子别名（Synonym）

IL1A,IL-1 ALPHA,IL1F1,IL1α

表达区间及表达系统（Source）

Biotinylated Human IL-1 alpha, His,Avitag is expressed from human 293 cells . It contains AA Ser 113 - Ala 271.

Predicted N-terminus: Ser 113

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 21.6 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！