Human IL-11 Protein, His Tag

Human IL-11 Protein, His Tag

分子别名（Synonym）

IL12,p70,Interleukin-12

表达区间及表达系统（Source）

Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag  is expressed from human 293 cells . It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A).

Predicted N-terminus: Ile 23 (IL12B) & Arg 23 (IL12A)

Request for sequence

蛋白结构（Molecular Characterization）

Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 38.3 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-12A is fused with flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 40-50 kDa (IL12B) and 32-35 kDa (IL12A) respectively due to glyosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性（Bioactivity）-ELISA

Immobilized Human IL-12 R beta 1, Fc Tag  at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tagwith a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

Immobilized Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag  at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate. can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL (Routinely tested).

 

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