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Human IL-18BP Protein, His Tag (MALS verified)

Human IL-18BP Protein, His Tag (MALS verified)
别名: IL18BP,IL18BPa,Tadekinig-alfa
储存条件: -20℃ 
Human IL-18BP Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILP-H5222-200ug 200ug 现货 2500.00 2500
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熔点:
密度:
储存条件: -20℃

 

Human IL-18BP Protein, His Tag (MALS verified)

分子别名(Synonym)

IL18BP,IL18BPa,Tadekinig-alfa

表达区间及表达系统(Source)

Human IL-18BP, His Tag  is expressed from human 293 cells. It contains AA Thr 31 - Gly 194 .

Predicted N-terminus: Thr 31

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.5 kDa. The protein migrates as 32-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-18BP, His Tag (Cat. No. ILP-H5222) SDS-PAGE gel

Human IL-18BP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human IL-18BP, His Tagis more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human IL-18BP, His Tag inhibits the IL-18-induced IFN-gamma secretion by KG‑1 cells. The ED50 for this effect is 0.019-0.053 µg/mL in the presence of 30 ng/mL of Recombinant Human IL‑18 (Routinely tested).

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