-
专业包装 正品保证
-
快乐服务 售后无忧
-
会员特权 优惠不断
-
个人信息 严格保护
| 别名: | IL12B,CLMF,CLMF2,NKSF,NKSF2,p40 | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human IL-12B / NKSF2 / p40 Protein, His Tag
IL12B,CLMF,CLMF2,NKSF,NKSF2,p40
Human IL-12B, His Tag is expressed from human 293 cells. It contains AA Ile 23 - Ser 328 .
Predicted N-terminus: Ile 23
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-12B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti IL23A & IL12B MAb at 2 μg/mL (100 μL/well) can bind Human IL-12B, His Tag with a linear range of 0.4-13 ng/mL (QC tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tag on Protein A Biosensor, can bind Human IL-12B, His Tag with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-IL23A & IL12B MAb (P40 domain) on Protein A Biosensor, can bind Human IL-12B, His Tag with an affinity constant of 0.279 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
