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Human IL-11 Protein, Fc Tag (MALS verified)

Human IL-11 Protein, Fc Tag (MALS verified)
别名: IL-11,Interleukin-11,AGIF,Oprelvekin,IL11
储存条件: -20℃ 
Human IL-11 Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IL1-H5269-20ug 20ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

 

Human IL-11 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL-11,Interleukin-11,AGIF,Oprelvekin,IL11

表达区间及表达系统(Source)

Human IL-11, Fc Tag is expressed from human 293 cells. It contains AA Pro 22 - Leu 199

Predicted N-terminus: Pro

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 45.6 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-11, Fc Tag (Cat. No. IL1-H5269) SDS-PAGE gel

Human IL-11, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IL-11, Fc Tag  is more than 95% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-11 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-11, Fc Tag with a linear range of 0.2-10 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human IL-11, Fc Tag  at 5 μg/mL (100 μL/well) can bind Human IL-11 R alpha, His Tag with a linear range of 0.008-0.5 μg/mL (Routinely tested).

 

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