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| 别名: | IL-23 alpha & IL-12 beta | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-23 alpha & Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)
IL-23 alpha & IL-12 beta
Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells It contains AA Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B).
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequence
Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The reducing (R) protein migrates as 20 kDa (IL-23 alpha) and 45-50 kDa (IL-12 beta) respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Immobilized Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Freeat 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Human IL-23 R, Fc Tag at 10 μg/mL (100 μL/well) can bind Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Freewith a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
Loaded Human IL-23 R, Fc Tag on Protein A Biosensor, can bind Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 10.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tag on Protein A Biosensor, can bind Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 2.79 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Freestimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.0069-0.0426 ng/mL (Routinely tested).
Protocol
Cell based assay shows that the secretion of IL-17 induced by Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free is inhibited by increasing concentration of the Anti-Human IL-23 neutralizing antibody. The IC50 is between 0.41-1.012 ng/mL (Routinely tested).
