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| 别名: | IL-23 alpha & IL-12 beta | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free
IL-23 alpha & IL-12 beta
Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells . It contains AA Val 22 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B) .
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequence
Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 20.3 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The reducing (R) protein migrates as 20-23 kDa (IL-23 alpha) and 40-42 kDa and 43-46 kDa (IL-12 beta) respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human IL-23 R, Fc,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 0.03-0.25 μg/mL (QC tested).
Protocol
Loaded Human IL-23 R, Fc Tagon Protein A Biosensor, can bind Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 9.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
