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| 别名: | IL6,Interleukin-6,BSF2,HSF,IFNB2 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
ActiveMax® Human IL-6 Protein, Tag Free
IL6,Interleukin-6,BSF2,HSF,IFNB2
ActiveMax® Human IL-6, Tag Free is expressed from human 293 cells. It contains AA Val 30 - Met 212 .
Predicted N-terminus: Val 30
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human IL-6, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax® Human IL-6, Tag Freeat 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Avitag,His Tag with a linear range of 4-125 ng/mL (QC tested).
Protocol
Loaded Human IL-6 R alpha, Fc Tag on Protein A Biosensor, can bind ActiveMax® Human IL-6, Tag Free with an affinity constant of 35.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
ActiveMax® Human IL-6, Tag Free stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2856-0.3636 ng/mL (Routinely tested).
