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Human Latent TGF-beta 2 / TGFB2 Protein, His Tag

Human Latent TGF-beta 2 / TGFB2 Protein, His Tag
别名: Latent TGFB2,MGC116892,TGFβ2,TGF-beta2
储存条件: -20℃ 
Human Latent TGF-beta 2 / TGFB2 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-TG2-H5243-50ug 50ug 现货 3350.00 3350
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熔点:
密度:
储存条件: -20℃

 Human Latent TGF-beta 2 / TGFB2 Protein, His Tag

分子别名(Synonym)

Latent TGFB2,MGC116892,TGFβ2,TGF-beta2

表达区间及表达系统(Source)

Human Latent TGFB2, His Tag is expressed from human 293 cells It contains AA Leu 21 - Ser 414 

 

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 47.6 kDa. The protein migrates as 12 kDa and 40-50 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human Latent TGFB2, His Tag (Cat. No. TG2-H5243) SDS-PAGE gel

Human Latent TGFB2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Latent TGFB2, His Tagat 5 μg/mL (100 μL/well) can bind Human LRRC32, Fc Tag with a linear range of 0.039-0.625 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human Latent TGFB2, His Tag at 5 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag with a linear range of 0.156-2.5 μg/mL (Routinely tested).

 

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