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| 别名: | CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human LIGHT / TNFSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin
CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin is expressed from human 293 cells. It contains AA Asp 74 - Val 240
Predicted N-terminus: Glu
Request for sequence
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 45.1 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Loaded Human DcR3, Fc Tagon AHC Biosensor, can bind Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 1.64 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
