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Cynomolgus IL-3 R alpha / CD123 Protein, His Tag (SPR verified)

Cynomolgus IL-3 R alpha / CD123 Protein, His Tag (SPR verified)
别名: IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha
储存条件: -20℃ 
Cynomolgus IL-3 R alpha / CD123 Protein, His Tag (SPR verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILA-C52H6-100ug 100ug 现货 30005.00 30005
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熔点:
密度:
储存条件: -20℃

 

Cynomolgus IL-3 R alpha / CD123 Protein, His Tag (SPR verified)

分子别名(Synonym)

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表达区间及表达系统(Source)

Cynomolgus IL-3 R alpha, His Tag (SPR verified) is expressed from human 293 cells . It contains AA Arg 18 - Arg 302 .

Predicted N-terminus: Arg 18

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 34.7 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Cynomolgus IL-3 R alpha, His Tag (SPR verified) (Cat. No. ILA-C52H6) SDS-PAGE gel

Cynomolgus IL-3 R alpha, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Cynomolgus IL-3 R alpha, His Tag (SPR verified) captured on CM5 Chip via anti-His antibody can bind Human IL-3, Tag Free with an affinity constant of 0.271 μM as determined in SPR assay (Biacore 8k) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Cynomolgus IL-3 R alpha, His Tag (SPR verified)  on NTA Biosensor, can bind Human IL-3, Tag Free with an affinity constant of 0.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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