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Mouse CD40 Ligand / TNFSF5 Protein, Fc Tag

Mouse CD40 Ligand / TNFSF5 Protein, Fc Tag
别名: CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
储存条件: -20℃ 
Mouse CD40 Ligand / TNFSF5 Protein, Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CDL-M526x-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

Mouse CD40 Ligand / TNFSF5 Protein, Fc Tag

分子别名(Synonym)

CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39

表达区间及表达系统(Source)

Mouse CD40 Ligand, Fc Tagis expressed from human 293 cells . It contains AA Gly 115 - Leu 260.

 

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 42.9 kDa. As a result of glycosylation, the protein migrates as 52 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse CD40 Ligand, Fc Tag (Cat. No. CDL-M526x) SDS-PAGE gel

Mouse CD40 Ligand, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse CD40, His Tag at 5 μg/mL (100 μL/well) can bind Mouse CD40 Ligand, Fc Tagwith a linear range of 1-16 ng/mL (QC tested).

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