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N-酰基鞘氨醇酰胺水解酶2(ASAH2)重组蛋白(Human,人)

Recombinant N-Acylsphingosine Amidohydrolase 2 (ASAH2)
别名: HNAC1; non-lysosomal ceramidase; Neutral ceramidase
储存条件: 在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。 
N-酰基鞘氨醇酰胺水解酶2(ASAH2)重组蛋白(Human,人)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY62516HuP-10μg 10μg 现货 840.00 840
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熔点:
密度:
储存条件: 在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。

N-酰基鞘氨醇酰胺水解酶2(ASAH2)重组蛋白

规格及价格:

规格 价格
10µg 840
50µg 2100
200µg 4200
1mg 12600
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Ala451~Ile780
Tags: Two N-terminal Tags, His-tag and SUMO-tag
Subcellular Location: Membrane, Secreted, Golgi apparatus
Purity: > 90% 
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 50.4kDa
Accurate Molecular Mass: 21&40&56kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation: 
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]

[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。
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