Anti-IL22 antibody

Anti-IL22 antibody

• 概述

• 产品描述Interleukin-22 (IL-22) is protein that in humans is encoded by the IL22 gene. IL-22 is produced by several populations of immune cells at a site of inflammation. Producers are αβ T cells classes Th1, Th22 and Th17 along with γδ T cells, NKT, ILC3, neutrophils and macrophages. IL-22 takes effect on non-hematopoietic cells – mainly stromal and epithelial cells. Effects involve stimulation of cell survival, proliferation and synthesis of antimicrobials including S100, Reg3β, Reg3γ and defensins. IL-22 thus participates in both wound healing and in protection against microbes. IL-22 dysregulation takes part in pathogenesis of several autoimmune diseases like systemic lupus erythematosus, rheumatoid arthritis and psoriasis. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). IL-22 and IL-10 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
• 产品名称Anti-IL22 antibody
• 分子量17 KDa
• 种属反应性Human,Mouse,Rat,Dog
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human IL-22 35-179/179
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式Affinity purified by Protein A
• 亚细胞定位Secreted.
• 其它名称Interleukin-22
  Il22
  IL-22
  Iltif
  ILTIFa
  MGC129416
  MGC129417
  IL-10-related T-cell-derived-inducible factor
  IL-TIF
  IL-TIF alpha
  Interleukin-22a
  IL-22a
  IL 21
  IL D110
  IL TIF
  IL21
  IL22
  ILD110
  interleukin 21
  MGC79382
  MGC79384
  TIFa
  TIFIL 23
  TIFIL23
  zcyto18.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg /test

•  

  

  Fig1: Sample:
  A549 (Human) Cell Lysate at 30 ug
  Primary: Anti-IL22 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 17 kD
  Observed band size: 19 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig3: Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
  Primary Antibody: Rabbit Anti-IL22 antibody  Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
  Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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